Relation between inflammation and growth and metastasis of Lewis lung carcinoma in process of serial passage in mice Lewis肺癌在小鼠体内传代过程中生长转移能力的演变与炎症的关系
Methods: Gastric carcinoma BGC-823 cells were cultured for serial passage and then treated with bFGF. 方法:胃癌BGC-823细胞传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage. 兔角膜上皮和内皮细胞组织块原代培养,消化接种于人胚肺饲细胞瓶传代培养。
A hemopoietic stroma cell strain from mouse fetal liver ( MFLSC) has been established through a serial primary passage and cloned culture. 小鼠胎肝造血基质细胞经原代和传代培养并克隆成细胞株。
It has practical significance for us to design lower energy loss pneumatic spool valves. The effective areas of the serial passage in one of the valve were calculated. It is found that the difference is 8.9% between different passages. 气动换向阀串联流道等效面积计算结果表明,不同通道之间的等效面积差别为8.9%,可见各流道结构参数没有得到合理匹配。
RT-PCR was used to detect the transcription of TGEV spike protein, enhanced green fluorescence ( eGFP) was used to test the expression of foreign protein, and serial passage and PCR were used to detect the stability and safety of rAd-S. 以RT-PCR法检测重组腺病毒rAd-S中TGEV纤突糖蛋白S基因的转录,增强型绿色荧光蛋白(eGFP)荧光检测外源基因的表达,用连续传代和PCR检测rAd-S的稳定性和安全性。
METHODS: Using the method of enzyme digested, Osteoblasts ( OB) obtained from newborn rat calvaria were cultured for serial passage. 方法:采用酶消化法从新生SD大鼠头盖骨分离成骨细胞,传代培养。
Studies on serial passage of bone marrow stromal cells in normal human in vitro 正常人骨髓基质祖细胞体外传代培养的实验研究
Serial Passage of Mamestra brassicae Nuclear Polyhedrosis Virus in Vitro 甘蓝夜蛾核型多角体病毒连续传代的研究
Xenotransplanted tumors and tumors after serial passage maintained the genetic stability. 异种移植瘤及其传代肿瘤保持遗传稳定性。
Methods Primary culture of human trophoblast cells was carried out by tissue-culture method, and the subsequent serial passage of the cells performed by trypsin digestion, identified and assayed by immunohistochemical staining. 方法用组织块法行原代培养,胰蛋白酶消化法行传代培养,免疫组织化学染色进行细胞鉴定。
The suspended stem cells may be purified by limited differentiation and serial passage in SFM. 使用干细胞培养液培养,并通过限制分化、连续传代的方法,可分离纯化出悬浮克隆集落生长的干细胞。
According to this theory, if the tumor cells, which have enhanced malignant potency after clonal selection by the mean of serial passage in mice, were purified and inoculated to the mices again, the growth speed and metastatic ability of the tumor cells should be enhanced. 根据该理论,如果我们将这种经过克隆选择、生长转移能力增强的肿瘤细胞提取出来,再次接种小鼠其生长转移能力也应该是增强的。